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mmp13 primary antibody  (Proteintech)


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    Proteintech mmp13 primary antibody
    Mmp13 Primary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mmp13 primary antibody/product/Proteintech
    Average 90 stars, based on 1 article reviews
    mmp13 primary antibody - by Bioz Stars, 2026-02
    90/100 stars

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    Effects of CIHH on the expression of <t>MMP13,</t> ADAMTS5 and COMP in cartilage in the PTOA model at timepoint of 7 days post-injury. A . Effect of CIHH on MMP13 and ADAMTS5 expression in PTOA mouse chondrocytes. The protein expression levels of MMP13 and ADAMTS5 were determined by Western blotting. B and C . Quantification of the expression levels of MMP13 and ADAMTS5 proteins in A respectively. D . Quantification of COMP-positive cells in cartilage in the PTOA model. E . Immunohistochemistry staining of COMP and MMP13. F . Immunofluorescence staining of collagen II and DAPI staining of nuclei in tibial plateau chondrocytes of PTOA mouse (scale bar: 50 μm). Black arrow: positive cells. J . The collagen-II fluorescence intensity was measured with ImageJ 2.9.0 software. The data in the figures are presented as the mean ± SEM ( n = 3). Significant differences among different groups are indicated as follows: ** P < 0.01, vs. the CON group; # P < 0.05 and ## P < 0.01, vs. the MS group. Scale bar = 50 μm
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    Image Search Results


    Arctiin’s role in counteracting IL-1β-induced inflammatory and ECM deterioration in chondrocytes. ( A , B ) Immunofluorescence imaging of Collagen II and MMP13 post-treatment; scale bar: 100 μm. ( C , D ) Fluorescence intensity quantification for Collagen II and MMP13 using ImageJ. ( E , F ) mRNA levels of COX-2, MMP3, MMP13, Collagen II and SOX9quantified via RT-PCR. ( G , H , I ) Protein expression and quantitative analysis of COX-2, MMP3, MMP13, Collagen II and SOX9, performed by Western blotting. Data are presented as means ± SD. Significant differences indicated by # P < 0.05, ## P < 0.01, ### P < 0.001 compared to control, and * P < 0.05, ** P < 0.01, *** P < 0.001 compared to the IL-1β-treated group; n = 3.

    Journal: Scientific Reports

    Article Title: Arctiin alleviates the progression of osteoarthritis by regulating the cholesterol metabolic pathway

    doi: 10.1038/s41598-024-83993-1

    Figure Lengend Snippet: Arctiin’s role in counteracting IL-1β-induced inflammatory and ECM deterioration in chondrocytes. ( A , B ) Immunofluorescence imaging of Collagen II and MMP13 post-treatment; scale bar: 100 μm. ( C , D ) Fluorescence intensity quantification for Collagen II and MMP13 using ImageJ. ( E , F ) mRNA levels of COX-2, MMP3, MMP13, Collagen II and SOX9quantified via RT-PCR. ( G , H , I ) Protein expression and quantitative analysis of COX-2, MMP3, MMP13, Collagen II and SOX9, performed by Western blotting. Data are presented as means ± SD. Significant differences indicated by # P < 0.05, ## P < 0.01, ### P < 0.001 compared to control, and * P < 0.05, ** P < 0.01, *** P < 0.001 compared to the IL-1β-treated group; n = 3.

    Article Snippet: The study utilized primary antibodies targeting MMP3, MMP13, COX-2, Collagen II, and SOX9, which Affinity Biosciences, OH, USA provided.

    Techniques: Immunofluorescence, Imaging, Fluorescence, Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot, Control

    Sequences of primers for quantitative real-time PCR analyses.

    Journal: Scientific Reports

    Article Title: Arctiin alleviates the progression of osteoarthritis by regulating the cholesterol metabolic pathway

    doi: 10.1038/s41598-024-83993-1

    Figure Lengend Snippet: Sequences of primers for quantitative real-time PCR analyses.

    Article Snippet: The study utilized primary antibodies targeting MMP3, MMP13, COX-2, Collagen II, and SOX9, which Affinity Biosciences, OH, USA provided.

    Techniques: Real-time Polymerase Chain Reaction

    Effects of CIHH on the expression of MMP13, ADAMTS5 and COMP in cartilage in the PTOA model at timepoint of 7 days post-injury. A . Effect of CIHH on MMP13 and ADAMTS5 expression in PTOA mouse chondrocytes. The protein expression levels of MMP13 and ADAMTS5 were determined by Western blotting. B and C . Quantification of the expression levels of MMP13 and ADAMTS5 proteins in A respectively. D . Quantification of COMP-positive cells in cartilage in the PTOA model. E . Immunohistochemistry staining of COMP and MMP13. F . Immunofluorescence staining of collagen II and DAPI staining of nuclei in tibial plateau chondrocytes of PTOA mouse (scale bar: 50 μm). Black arrow: positive cells. J . The collagen-II fluorescence intensity was measured with ImageJ 2.9.0 software. The data in the figures are presented as the mean ± SEM ( n = 3). Significant differences among different groups are indicated as follows: ** P < 0.01, vs. the CON group; # P < 0.05 and ## P < 0.01, vs. the MS group. Scale bar = 50 μm

    Journal: Journal of Orthopaedic Surgery and Research

    Article Title: Chronic intermittent hypobaric hypoxia alleviates early-stage posttraumatic osteoarthritis via NF-κB/Nrf2 pathway in mice

    doi: 10.1186/s13018-024-05376-6

    Figure Lengend Snippet: Effects of CIHH on the expression of MMP13, ADAMTS5 and COMP in cartilage in the PTOA model at timepoint of 7 days post-injury. A . Effect of CIHH on MMP13 and ADAMTS5 expression in PTOA mouse chondrocytes. The protein expression levels of MMP13 and ADAMTS5 were determined by Western blotting. B and C . Quantification of the expression levels of MMP13 and ADAMTS5 proteins in A respectively. D . Quantification of COMP-positive cells in cartilage in the PTOA model. E . Immunohistochemistry staining of COMP and MMP13. F . Immunofluorescence staining of collagen II and DAPI staining of nuclei in tibial plateau chondrocytes of PTOA mouse (scale bar: 50 μm). Black arrow: positive cells. J . The collagen-II fluorescence intensity was measured with ImageJ 2.9.0 software. The data in the figures are presented as the mean ± SEM ( n = 3). Significant differences among different groups are indicated as follows: ** P < 0.01, vs. the CON group; # P < 0.05 and ## P < 0.01, vs. the MS group. Scale bar = 50 μm

    Article Snippet: Primary antibodies against MMP13 (GTX100665) were purchased from GeneTex, Inc. (Alton Pkwy Irvine, USA).

    Techniques: Expressing, Western Blot, Immunohistochemistry, Staining, Immunofluorescence, Fluorescence, Software