mmp13 primary antibody  (Proteintech)

Journal: bioRxiv

Article Title: Evaluating the Therapeutic Efficacy of Iopanoic Acid in a DMM-Induced Osteoarthritis Mouse Model and Osteochondral Lesioned Human Explants

doi: 10.1101/2025.07.15.663868

Figure Lengend Snippet: ( A ) Representative IHC images of COl2, MMP13, and CCDC80 staining in meniscus lesions of the knee joint. ( B ) Quantification of IHC staining intensity for COl2, MMP13, and CCDC80. ( C ) Multivariate analyses of COl2, MMP13 and CCDC80 using GEE to understand the independent therapeutic effect of IOP and hydrogel in the meniscus lesions. All data are shown as means ± standard deviations (n= 6). * P ≤0.05, **** P ≤0.0001. GEE: Generalized Estimating Equation.

Article Snippet: Primary MMP13 antibody incubation was done overnight at 4 C with 2 μg/mL MMP13 monoclonal antibody (SC-515284, Santa Cruz Biotechnology, Santa Cruz, Dallas, TX, USA) or with 2μg/mL normal mouse IgG1 as isotype control (SC-3877, Santa Cruz, Dallas, TX, USA).

Techniques: Staining, Immunohistochemistry

Journal: Scientific Reports

Article Title: Arctiin alleviates the progression of osteoarthritis by regulating the cholesterol metabolic pathway

doi: 10.1038/s41598-024-83993-1

Figure Lengend Snippet: Arctiin’s role in counteracting IL-1β-induced inflammatory and ECM deterioration in chondrocytes. ( A , B ) Immunofluorescence imaging of Collagen II and MMP13 post-treatment; scale bar: 100 μm. ( C , D ) Fluorescence intensity quantification for Collagen II and MMP13 using ImageJ. ( E , F ) mRNA levels of COX-2, MMP3, MMP13, Collagen II and SOX9quantified via RT-PCR. ( G , H , I ) Protein expression and quantitative analysis of COX-2, MMP3, MMP13, Collagen II and SOX9, performed by Western blotting. Data are presented as means ± SD. Significant differences indicated by # P < 0.05, ## P < 0.01, ### P < 0.001 compared to control, and * P < 0.05, ** P < 0.01, *** P < 0.001 compared to the IL-1β-treated group; n = 3.

Article Snippet: The study utilized primary antibodies targeting MMP3, MMP13, COX-2, Collagen II, and SOX9, which Affinity Biosciences, OH, USA provided.

Techniques: Immunofluorescence, Imaging, Fluorescence, Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot, Control

Journal: Scientific Reports

Article Title: Arctiin alleviates the progression of osteoarthritis by regulating the cholesterol metabolic pathway

doi: 10.1038/s41598-024-83993-1

Figure Lengend Snippet: Sequences of primers for quantitative real-time PCR analyses.

Article Snippet: The study utilized primary antibodies targeting MMP3, MMP13, COX-2, Collagen II, and SOX9, which Affinity Biosciences, OH, USA provided.

Techniques: Real-time Polymerase Chain Reaction

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Chronic intermittent hypobaric hypoxia alleviates early-stage posttraumatic osteoarthritis via NF-κB/Nrf2 pathway in mice

doi: 10.1186/s13018-024-05376-6

Figure Lengend Snippet: Effects of CIHH on the expression of MMP13, ADAMTS5 and COMP in cartilage in the PTOA model at timepoint of 7 days post-injury. A . Effect of CIHH on MMP13 and ADAMTS5 expression in PTOA mouse chondrocytes. The protein expression levels of MMP13 and ADAMTS5 were determined by Western blotting. B and C . Quantification of the expression levels of MMP13 and ADAMTS5 proteins in A respectively. D . Quantification of COMP-positive cells in cartilage in the PTOA model. E . Immunohistochemistry staining of COMP and MMP13. F . Immunofluorescence staining of collagen II and DAPI staining of nuclei in tibial plateau chondrocytes of PTOA mouse (scale bar: 50 μm). Black arrow: positive cells. J . The collagen-II fluorescence intensity was measured with ImageJ 2.9.0 software. The data in the figures are presented as the mean ± SEM ( n = 3). Significant differences among different groups are indicated as follows: ** P < 0.01, vs. the CON group; # P < 0.05 and ## P < 0.01, vs. the MS group. Scale bar = 50 μm

Article Snippet: Primary antibodies against MMP13 (GTX100665) were purchased from GeneTex, Inc. (Alton Pkwy Irvine, USA).

Techniques: Expressing, Western Blot, Immunohistochemistry, Staining, Immunofluorescence, Fluorescence, Software